Figure 5

Figure 5

P-HAP Protected Zebrafish Larvae Against Apoptosis and Reduced Inflammation After Alcohol Administration. (A)In situ detection of cell apoptosis by TUNEL staining of paraffin liver sections in control zebrafish larvae and zebrafish larvae treated with 350 mM ethanol or 50 μM p-HAP. Apoptotic cells are indicated by white arrowheads. Figures are magnified as 400×. (B) NF-κB immunohistochemical staining of zebrafish larvae. Figures are magnified as 400×. (C) Quantitative analysis of NF-κB expression. (D) The amounts of NF-κB expression were quantified according to fluorescence intensity. (E) NF-κB immunofluorescence staining of LO₂ slides. Figures are magnified as 200×. (F) Western blot analysis of caspase-9 expression in LO₂ treated with 350 mM ethanol and 50 μM p-HAP. (G) Quantitative analysis of caspase-9 protein expression. Data are expressed as the mean ± SD. ^###P < 0.0001 vs control group. ***P < 0.001 vs model group.