ZFIN Image: Huang et al., 2020, Figure 2

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Figure Caption

Figure 2

P-HAP attenuated hepatic steatosis induced by alcohol in zebrafish larvae. (A) H&E staining of the liver in zebrafish larva. Figures are magnified as 400×. (B) Lipid droplets in the whole-mount zebrafish liver were stained with oil red O after P-HAP treatment. Figures are magnified as 50×. (C) Oil red O staining of zebrafish larvae treated with 350 mM ethanol, 0.1% DMSO, and 25 μM, 50 μM, or 100 μM p-HAP and control zebrafish larvae were quantitatively analyzed. (D) The frozen liver sections from zebrafish larvae treated with 50 μM p-HAP were stained with Nile red. Figures are magnified as 200×. (E) Quantitative analysis of Nile red staining in zebrafish larvae. Data are expressed as the mean ± SD, n = 10 per group from two experiments using one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test; ^##P < 0.01, ^###P < 0.001 vs control group, ***P < 0.001 vs model group.

Acknowledgments

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