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Iribarne et al., 2019 - TNFα Induces Müller Glia to Transition From Non-proliferative Gliosis to a Regenerative Response in Mutant Zebrafish Presenting Chronic Photoreceptor Degeneration

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Fig. 1

Retinal apoptosis occurs transiently at 2–3 wpf and ceases by 5 wpf in gosh mutants. TUNEL was assessed on retinal slides of wild-type sibling (A,D,G,J,M) and gosh mutant retinas (B,E,H,K,N) at 2, 3, 5, 7, and 12 wpf. Apoptotic cells in the ONL are indicated by arrows. The transgenic line Tg(gnat2:GFP) is used to label cone photoreceptors. Nuclei are counterstained with TO-PRO-3. Wild-type sibling retinas show very low numbers of TUNEL-labeled cells at all stages (A,D,G,J,M). In gosh mutants, numbers of apoptotic cells are higher in the ONL at 2–3 wpf and in the INL at 2 wpf (B,E, insets show TUNEL⁺ cells in the ONL). Quantification of TUNEL-positive nuclei in the ONL and INL was performed (C,F,I,L,O). Bars and lines indicate means ± SEM, n: 3–7. Black and red bars: wild-type sibling and gosh mutants. Central retina of control and gosh retinas at 12 wpf show similar rod layer thickness, but cone photoreceptor shows a reduce number of cones (P–R, control left bars, gosh right bars) (ns, p > 0.05; ^∗p < 0.05; and ^∗∗∗p < 0.001). ONL, outer nuclear layer; INL, inner nuclear layer.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol