klf17-deficient embryos failed to hatch during zebrafish embryogenesis. (a,b) Hatching-deficient phenotype in the klf17-deficient embryos at 3 dpf (a) and 6 dpf (b). The klf17-deficient embryo (klf17uy21/uy22) failed to hatch at 3 and 6 dpf, whereas wild-type (klf17+/uy22) embryo hatched until 3 dpf. Scale bar, 1 mm. (c–e) Dechorionated-klf17-deficient embryos were alive at 15 dpf. Wild-type embryo (klf17+/+) (c) and the dechorionated- klf17-deficient embryo (klf17uy21/uy22) (d) were both alive at 15 dpf, whereas the klf17-deficient embryo (klf17uy21/uy22) (e) died without hatching. Scale bar, 2 mm. After taking pictures, genotyping of individual embryos was performed by genomic PCR.
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