Fig. 5

Fig. 5

Plasma membrane sustenance of Cxcr2 is required for neutrophil dispersal. a Confocal projections of neutrophil distribution in Tg(lyz:Cxcr2-WT-FT)/cxcr2^−/− larvae, Tg(lyz:Cxcr2-ala-FT)/cxcr2^−/−, and Tg(lyz:Cxcr2-chim-FT)/cxcr2^−/− at ~2 hpw. Dashed line indicates occupied wound area (owa). CHT: caudal hematopoietic tissue, VF: ventral fin, W: wound. Scale bar = 32 µm. b Neutrophil speed in relation to distance from the owa. Average speeds per cell per distance bin are shown. n = 558–2651 steps per bin (WT), n = 95–1266 steps per bin (ala), n = 494–2000 steps per bin (chim), and n = 1168–2823 steps per bin for (cxcr2^−/−). c Net reverse traffic. n = 6 (WT), n = 9 (ala), n = 8 (chim), n = 11 (−; cxcr2^−/−) larvae. Kruskal–Wallis test with Dunn’s multiple comparisons test. In b and c, data are from 6 (WT), 9 (ala), 8 (chim), and 11 (−; cxcr2^−/−) larvae from 3, 4, 3, and 8 imaging sessions, respectively. Cells were analyzed from the start of the movie (~15 mpw) up to 2 hpw. Error bars represent S.E.M. across cell steps (b) or larvae (c). Source data are provided as a Source Data file