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Fig. 4.

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ZDB-IMAGE-191230-524

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Publication

Ding et al., 2019 - Haploinsufficiency of mechanistic target of rapamycin ameliorates bag3 cardiomyopathy in adult zebrafish

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Figures for Ding et al., 2019

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Figure Caption

Fig. 4.

Transcriptome analysis identifies mTOR as one of the top signaling pathways affected in the bag3^e2/e2 mutant. (A) Numbers of differentially expressed (DE) genes between bag3^e2/e2 mutant hearts and WT control with a cutoff of adjusted P<0.05. (B) Heat map presenting the expression of 5361 DE genes between the bag3^e2/e2 mutant and WT control. Gene expression levels are shown in log₁₀ [reads per kilobase per million reads (RPKM)]. (C) The top 10 DE gene enriched pathways with significant P-values suggested by IPA. The red column shows that the mTOR pathway was one of the top pathways altered and was subsequently experimentally tested. (D) Representative western blot images and quantification analysis of the expression levels of mTOR downstream proteins. LC3-II protein was examined in heart tissues from fish treated with 50 nM bafilomycin A1 for 4 h. n=4 biological replicates, Student's t-test. Data are mean±s.e.m.

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