ZFIN Image: Wierson et al., 2019, FIG. 5.

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Figure Caption

FIG. 5.

Using pMini-CAAGs::RFP-DR48 to assay the propensity of ErCas12a and SpCas9 to elicit strand annealing in HEK293T cells. (A) Schematic showing the workflow for determining SAMR in human cells. An all-in-one expression plasmid for ErCas12a or SpCas9 is transfected along with pMini-CAAGs::RFP-DR48. Cells are allowed to recover and are then isolated with fluorescence-activated cell sorting for RFP+ cells. (B) Quantification of RFP+ cells per total cells analyzed by flow cytometry. Data plot represents the mean ± SD. p-Values calculated with one-tailed Student's t-test.

Acknowledgments

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