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Figure 1 supplement 2.

ID: ZDB-IMAGE-191230-1796
Publication: Gancz et al., 2019 - Distinct origins and molecular mechanisms contribute to lymphatic formation during cardiac growth and regeneration
All Figures: Figures for Gancz et al., 2019

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Figure Caption

Figure 1 supplement 2. <italic>Prox1a</italic> transgene labels cardiac lymphatics as well as a subset of coronary vessels.

(a) Lymphatics associated with cardiac fat tissue (yellow arrow), in 24 wpf (fish size 29 mm) Tg(mrc1a:EGFP);Tg(lyve1b:dsRed2) animals presented in Figure 1g. Light sheet image super imposed on a bright-field image (n = 3). (b) prox1a transgene is detected in blood vessels labeled by the flt1_9a arterial marker and highlighted following intravascular injection of Qdot705 (blue) (n = 4) (insets). (c) intravascularly injected Qdot705 is detected in blood capillaries labeled by prox1a only (yellow arrows), but not in double-positive flt4;prox1a lymphatic vessels (white arrows). (d,e) Prox1 immunostaining (green) confirms Prox1 expression in OFT lymphatics (d, inset, arrows) but not in prox1a transgene labeled (red) blood vessels (e, inset, yellow arrows). Erythrocyte nuclei inside prox1a-positive blood vessels are labeled by DAPI (blue). Prox1 also labels cardiomyocytes (n = 3). Scale bars are 200 µm. Anterior view in a,b,c,e, lateral view in d.

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