Fig. 3
- ID
- ZDB-IMAGE-180306-8
- Genes
- Publication
- Stones et al., 2017 - Zebrafish (Danio rerio) as a Vertebrate Model Host To Study Colonization, Pathogenesis, and Transmission of Foodborne Escherichia coli O157.
- All Figures
- Figures for Stones et al., 2017
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Fig. 3
EHEC triggers transient neutrophil recruitment to the intestinal region and surrounding tissue upon colonization. mpo::gfp zebrafish larvae were exposed to 108 CFU/ml foodborne EHEC::mCherry for 2 h, washed, mounted live in low-melting-temperature agarose-containing tricaine, and imaged for 12 h at 32°C. (A) Representative frames showing intestinal tract of infected fish after 0 h (Ai), 4 h (Aii), 8 h (Aiii), and 12 h (Aiv). Accumulation of fecal matter at the anal opening is also visible. (B) Neutrophils in the intestinal region and surrounding tissue (counts from the intestinal region as shown in panel A) were enumerated. Individual data points from infected fish (gray) and uninfected fish (blue), means (n = 5 per condition), and SD are shown. The statistical significance of results of comparisons between infected and uninfected fish for individual time points was determined using Student’s t test (*, P < 0.05; **, P < 0.01). ns, not significant.