Gene
lig4
- ID
- ZDB-GENE-070820-10
- Name
- ligase IV, DNA, ATP-dependent
- Symbol
- lig4 Nomenclature History
- Previous Names
-
- zgc:165595 (1)
- Type
- protein_coding_gene
- Location
- Chr: 1 Mapping Details/Browsers
- Description
- Predicted to enable ATP binding activity; DNA binding activity; and DNA ligase (ATP) activity. Acts upstream of or within double-strand break repair via nonhomologous end joining. Predicted to be located in nucleus. Predicted to be part of DNA ligase IV complex and DNA-dependent protein kinase-DNA ligase 4 complex. Human ortholog(s) of this gene implicated in several diseases, including DNA ligase IV deficiency; colorectal cancer; multiple myeloma; pancreatic cancer; and prostate cancer. Orthologous to human LIG4 (DNA ligase 4).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 2 figures from 2 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| fh302 | Allele with one point mutation | Unknown | Unknown | ENU | |
| fh303 | Allele with one point mutation | Unknown | Unknown | ENU | |
| lig4_unrecovered | Allele with one point mutation | Unknown | Unknown | ENU | |
| mhn7 | Allele with one deletion | Unknown | Unknown | CRISPR | |
| sa13669 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa32694 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa38268 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| zko558a | Allele with one deletion | Unknown | Unknown | CRISPR | |
| zko558b | Allele with one deletion | Unknown | Unknown | CRISPR |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-lig4 | Feng et al., 2016 | |
| CRISPR2-lig4 | (2) | |
| CRISPR3-lig4 | Carrara et al., 2025 | |
| CRISPR4-lig4 | Carrara et al., 2025 | |
| MO1-lig4 | N/A | (4) |
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Human Disease
| Disease Ontology Term | Multi-Species Data | OMIM Term | OMIM Phenotype ID |
|---|---|---|---|
| DNA ligase IV deficiency | Alliance | LIG4 syndrome | 606593 |
| multiple myeloma | Alliance | {Multiple myeloma, resistance to} | 254500 |
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Domain, Family, and Site Summary
| Type | InterPro ID | Name |
|---|---|---|
| Conserved_site | IPR016059 | DNA ligase, ATP-dependent, conserved site |
| Domain | IPR001357 | BRCT domain |
| Domain | IPR012308 | DNA ligase, ATP-dependent, N-terminal |
| Domain | IPR012309 | DNA ligase, ATP-dependent, C-terminal |
| Domain | IPR012310 | DNA ligase, ATP-dependent, central |
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Domain Details Per Protein
| Protein | Additional Resources | Length | BRCT domain | BRCT domain superfamily | DNA ligase 4 | DNA Ligase 4, adenylation domain | DNA ligase, ATP-dependent | DNA ligase, ATP-dependent, central | DNA ligase, ATP-dependent, conserved site | DNA ligase, ATP-dependent, C-terminal | DNA ligase, ATP-dependent, N-terminal | DNA ligase, ATP-dependent, N-terminal domain superfamily | DNA ligase IV domain | Nucleic acid-binding, OB-fold |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:A7E283 | InterPro | 909 | ||||||||||||
| UniProtKB:F1Q9E8 | InterPro | 909 |
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Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-198K9 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:165595 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001103123 (1) | 3084 nt | ||
| Genomic | GenBank:BX908780 (1) | 199679 nt | ||
| Polypeptide | UniProtKB:A7E283 (1) | 909 aa |
- Carrara, M., Gaillard, A.L., Brion, A., Duvernois-Berthet, E., Giovannangeli, C., Concordet, J.P., Pézeron, G. (2025) Dynamic interplay of cNHEJ and MMEJ pathways of DNA double-strand break repair during embryonic development in zebrafish. Scientific Reports. 15:48864886
- Bian, W.P., Chen, Y., Luo, J., Wang, C., Xie, S., Pei, D. (2019) A knock-in strategy for editing human and zebrafish mitochondrial DNA using mito-CRISPR/Cas9 system. ACS synthetic biology. 8(4):621-632
- Honjo, Y., Ichinohe, T. (2019) Cellular responses to ionizing radiation change quickly over time during early development in zebrafish. Cell biology international. 43(5):516-527
- Sun, Y., Zhang, B., Luo, L., Shi, D.L., Wang, H., Cui, Z., Huang, H., Cao, Y., Shu, X., Zhang, W., Zhou, J., Li, Y., Du, J., Zhao, Q., Chen, J., Zhong, H., Zhong, T.P., Li, L., Xiong, J.W., Peng, J., Xiao, W., Zhang, J., Yao, J., Yin, Z., Mo, X., Peng, G., Zhu, J., Chen, Y., Zhou, Y., Liu, D., Pan, W., Zhang, Y., Ruan, H., Liu, F., Zhu, Z., Meng, A., ZAKOC Consortium (2019) Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9. Genome research. 30(1):118-26
- Won, M., Dawid, I.B. (2017) PCR artifact in testing for homologous recombination in genomic editing in zebrafish. PLoS One. 12:e0172802
- Feng, Y., Chen, C., Han, Y., Chen, Z., Lu, X., Liang, F., Li, S., Qin, W., Lin, S. (2016) Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9. G3 (Bethesda). 6(8):2517-21
- Thyme, S.B., Schier, A.F. (2016) Polq-Mediated End Joining Is Essential for Surviving DNA Double-Strand Breaks during Early Zebrafish Development. Cell Reports. 15(4):707-714
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Gong, L., Gong, H., Pan, X., Chang, C., Ou, Z., Ye, S., Yin, L., Yang, L., Tao, T., Zhang, Z., Liu, C., Lane, D.P., Peng, J., Chen, J. (2015) p53 isoform Δ113p53/Δ133p53 promotes DNA double-strand break repair to protect cell from death and senescence in response to DNA damage. Cell Research. 25(3):351-69
- He, M.D., Zhang, F.H., Wang, H.L., Wang, H.P., Zhu, Z.Y., Sun, Y.H. (2015) Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos. Mutation research. 780:86-96
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