Gene
tmie
- ID
- ZDB-GENE-061207-9
- Name
- transmembrane inner ear
- Symbol
- tmie Nomenclature History
- Previous Names
-
- si:ch211-163f10.1
- Type
- protein_coding_gene
- Location
- Chr: 2 Mapping Details/Browsers
- Description
- Acts upstream of or within inner ear morphogenesis; lateral line development; and sensory perception of sound. Predicted to be located in membrane. Is expressed in brain and otic vesicle. Human ortholog(s) of this gene implicated in autosomal recessive nonsyndromic deafness 6 and sensorineural hearing loss. Orthologous to human TMIE (transmembrane inner ear).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 4 figures from 4 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
- All Phenotype Data
- 14 figures from 4 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| ihb581 | Allele with one deletion | Exon 3 | Unknown | CRISPR | |
| ihb582 | Allele with one deletion | Exon 3 | Unknown | CRISPR | |
| la014848Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| ru1000 | Allele with one delins | Unknown | Unknown | not specified | |
| t26171 | Allele with one point mutation | Unknown | Unknown | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-tmie | Varshney et al., 2015 | |
| CRISPR2-tmie | (2) | |
| CRISPR3-tmie | (3) | |
| CRISPR4-tmie | Zebrafish Nomenclature Committee | |
| MO1-tmie | N/A | Shen et al., 2008 |
| MO2-tmie | N/A | Shen et al., 2008 |
| MO3-tmie | N/A | Gleason et al., 2009 |
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Human Disease
| Disease Ontology Term | Multi-Species Data | OMIM Term | OMIM Phenotype ID |
|---|---|---|---|
| autosomal recessive nonsyndromic deafness 6 | Alliance | Deafness, autosomal recessive 6 | 600971 |
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Domain, Family, and Site Summary
| Type | InterPro ID | Name |
|---|---|---|
| Family | IPR032006 | Transmembrane inner ear expressed protein |
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Domain Details Per Protein
| Protein | Additional Resources | Length | Transmembrane inner ear expressed protein |
|---|---|---|---|
| UniProtKB:D2ITC5 | InterPro | 231 | |
| UniProtKB:A2AVI6 | InterPro | 175 | |
| UniProtKB:A0A8M9Q9E3 | InterPro | 125 |
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Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg(myo6b:gria2a-tmie-2A-NLS-mCherry) |
| 1 | Pacentine et al., 2019 | ||
| Tg(myo6b:gria2a-tmie-EGFP) |
| 1 | Pacentine et al., 2019 | ||
| Tg(myo6b:tmie-2A-NLS-mCherry) |
| 1 | Pacentine et al., 2019 | ||
| Tg(myo6b:tmie-EGFP) |
|
| 1 | (2) | |
| Tg(myo6b:tmie-HA) |
|
| 1 | Erickson et al., 2017 | |
| Tg(myo6b:tmie-Hsa.CD8A-2A-NLS-mCherry) |
| 1 | Pacentine et al., 2019 | ||
| Tg(myo6b:tmie-Hsa.CD8A-EGFP) |
|
| 1 | Pacentine et al., 2019 |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH73-264D1 | ZFIN Curated Data | |
| Contained in | BAC | CH211-163F10 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001170648 (1) | 696 nt | ||
| Genomic | GenBank:CU929426 (2) | 107636 nt | ||
| Polypeptide | UniProtKB:D2ITC5 (1) | 231 aa |
- Jędrychowska, J., Vardanyan, V., Wieczor, M., Marciniak, A., Czub, J., Amini, R., Jain, R., Shen, H., Choi, H., Kuznicki, J., Korzh, V. (2024) Mutant analysis of Kcng4b reveals how the different functional states of the voltage-gated potassium channel regulate ear development. Developmental Biology. 513:50-62
- Sun, P., Smith, E., Nicolson, T. (2023) Transmembrane channel-like (Tmc) subunits contribute to frequency sensitivity in the zebrafish utricle. The Journal of neuroscience : the official journal of the Society for Neuroscience. 44(1):
- Smith, E.T., Pacentine, I., Shipman, A., Hill, M., Nicolson, T. (2020) Disruption of tmc1/2a/2b genes in zebrafish reveals subunit requirements in subtypes of inner ear hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 40(23):4457-4468
- Pacentine, I.V., Nicolson, T. (2019) Subunits of the mechano-electrical transduction channel, Tmc1/2b, require Tmie to localize in zebrafish sensory hair cells. PLoS Genetics. 15:e1007635
- Erickson, T., Morgan, C.P., Olt, J., Hardy, K., Busch-Nentwich, E.M., Maeda, R., Clemens-Grisham, R., Krey, J.F., Nechiporuk, A.V., Barr-Gillespie, P.G., Marcotti, W., Nicolson, T. (2017) Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt). eLIFE. 6
- Carrington, B., Varshney, G.K., Burgess, S.M., Sood, R. (2015) CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity. Nucleic acids research. 43(22):e157
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Varshney, G.K., Pei, W., LaFave, M.C., Idol, J., Xu, L., Gallardo, V., Carrington, B., Bishop, K., Jones, M., Li, M., Harper, U., Huang, S.C., Prakash, A., Chen, W., Sood, R., Ledin, J., Burgess, S.M. (2015) High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9. Genome research. 25(7):1030-42
- Pujol-Martí, J., Faucherre, A., Aziz-Bose, R., Asgharsharghi, A., Colombelli, J., Trapani, J.G., López-Schier, H. (2014) Converging Axons Collectively Initiate and Maintain Synaptic Selectivity in a Constantly Remodeling Sensory Organ. Current biology : CB. 24(24):2968-74
- Hu, Z.Y., Zhang, Q.Y., Qin, W., Tong, J.W., Zhao, Q., Han, Y., Meng, J., and Zhang, J.P. (2013) Gene miles-apart is required for formation of otic vesicle and hair cells in zebrafish. Cell Death & Disease. 4:e900
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