Gene
rock2b
- ID
- ZDB-GENE-060125-2
- Name
- rho-associated, coiled-coil containing protein kinase 2b
- Symbol
- rock2b Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 20 Mapping Details/Browsers
- Description
- Predicted to enable Rho-dependent protein serine/threonine kinase activity. Acts upstream of or within Kupffer's vesicle development; epithelial cilium movement involved in determination of left/right asymmetry; and heart morphogenesis. Predicted to be located in nucleus and plasma membrane. Predicted to be active in centrosome and cytoplasm. Is expressed in several structures, including EVL; Kupffer's vesicle; forerunner cell group; hindbrain; and somite. Orthologous to human ROCK2 (Rho associated coiled-coil containing protein kinase 2).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 8 figures from 3 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- cb64 (16 images)
Wild Type Expression Summary
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| la021576Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| la021577Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| la021578Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| sa17708 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa23748 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa23749 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa29396 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa43480 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa43481 | Allele with one point mutation | Unknown | Premature Stop | ENU |
1 - 9 of 9
Show
| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| MO1-rock2b | N/A | (5) |
| MO2-rock2b | N/A | Wang et al., 2011 |
| MO3-rock2b | N/A | Lisowska et al., 2018 |
1 - 3 of 3
Show
Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | AGC-kinase, C-terminal | C1-like domain superfamily | PH-like domain superfamily | Pleckstrin homology domain | Protein kinase, ATP binding site | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain | Protein kinase, C-terminal | Protein kinase domain | Protein kinase-like domain superfamily | Rho-associated protein kinase 1/2 | Rho-associated protein kinase 2, HR1 domain | Rho-associated Serine/Threonine Kinase | ROCK, Rho binding domain | Serine/threonine-protein kinase, active site |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:A0A8M1NDD8 | InterPro | 1401 | ||||||||||||||
| UniProtKB:A0A8M6YUR0 | InterPro | 1468 | ||||||||||||||
| UniProtKB:A0A8M3AT74 | InterPro | 941 | ||||||||||||||
| UniProtKB:A0A8M9PP57 | InterPro | 1346 | ||||||||||||||
| UniProtKB:A0A8M9PP53 | InterPro | 1493 |
1 - 5 of 13 Show all
| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
rock2b-201
(1)
|
Ensembl | 735 nt | ||
| mRNA |
rock2b-204
(1)
|
Ensembl | 4,206 nt | ||
| mRNA |
rock2b-206
(1)
|
Ensembl | 5,158 nt | ||
| ncRNA |
rock2b-003
(1)
|
Ensembl | 801 nt | ||
| ncRNA |
rock2b-004
(1)
|
Ensembl | 767 nt |
1 - 5 of 6 Show all
Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-199M3 | ZFIN Curated Data | |
| Contained in | BAC | DKEY-65B13 | ZFIN Curated Data | |
| Contained in | Fosmid | CH1073-370O3 | ZFIN Curated Data | |
| Encodes | EST | cb64 | Thisse et al., 2001 | |
| Encodes | EST | wz13503 | ZFIN Curated Data |
1 - 5 of 5
Show
| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001093747 (1) | 4206 nt | ||
| Genomic | GenBank:AL929220 (2) | 227539 nt | ||
| Polypeptide | UniProtKB:A0A8M9PP53 (1) | 1493 aa |
- Comparative Orthology
- Alliance
- Ferreira, R.R., Pakula, G., Klaeyle, L., Fukui, H., Vilfan, A., Supatto, W., Vermot, J. (2018) Chiral Cilia Orientation in the Left-Right Organizer. Cell Reports. 25:2008-2016.e4
- Lisowska, J., Rödel, C.J., Manet, S., Miroshnikova, Y.A., Boyault, C., Planus, E., De Mets, R., Lee, H.H., Destaing, O., Mertani, H., Boulday, G., Tournier-Lasserve, E., Balland, M., Abdelilah-Seyfried, S., Albiges-Rizo, C., Faurobert, E. (2018) Cerebral Cavernous Malformation 1/2 complex controls ROCK1 and ROCK2 complementary functions for endothelial integrity. Journal of Cell Science. 131(15)
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Lahne, M., Li, J., Marton, R.M., Hyde, D.R. (2015) Actin-Cytoskeleton- and Rock-Mediated INM Are Required for Photoreceptor Regeneration in the Adult Zebrafish Retina. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35:15612-34
- Varshney, G.K., Lu, J., Gildea, D., Huang, H., Pei, W., Yang, Z., Huang, S.C., Schoenfeld, D.S., Pho, N., Casero, D., Hirase, T., Mosbrook-Davis, D.M., Zhang, S., Jao, L.E., Zhang, B., Woods, I.G., Zimmerman, S., Schier, A.F., Wolfsberg, T., Pellegrini, M., Burgess, S.M., and Lin, S. (2013) A large-scale zebrafish gene knockout resource for the genome-wide study of gene function. Genome research. 23(4):727-735
- Wang, G., Yost, H.J., and Amack, J.D. (2013) Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle. Journal of visualized experiments : JoVE. (73)
- Winata, C.L., Kondrychyn, I., Kumar, V., Srinivasan, K.G., Orlov, Y., Ravishankar, A., Prabhakar, S., Stanton, L.W., Korzh, V., and Mathavan, S. (2013) Genome wide analysis reveals zic3 interaction with distal regulatory elements of stage specific developmental genes in zebrafish. PLoS Genetics. 9(10):e1003852
- Chu, L.T., Fong, S.H., Kondrychyn, I., Loh, S.L., Ye, Z., and Korzh, V. (2012) Yolk syncytial layer formation is a failure of cytokinesis mediated by Rock1 function in the early zebrafish embryo. Biology Open. 1(8):747-753
- Wang, G., Manning, M.L., and Amack, J.D. (2012) Regional Cell Shape Changes Control Form and Function of Kupffer's Vesicle in the Zebrafish Embryo. Developmental Biology. 370(1):52-62
- Chung, A.Y., Kim, S., Kim, H., Bae, Y.K., and Park, H.C. (2011) Microarray Screening for Genes Involved in Oligodendrocyte Differentiation in the Zebrafish CNS. Experimental Neurobiology. 20(2):85-91
1 - 10 of 12
Show