Gene
myo5b
- ID
- ZDB-GENE-031219-7
- Name
- myosin VB
- Symbol
- myo5b Nomenclature History
- Previous Names
-
- cb1077 (1)
- gsp
- si:ch73-112l6.5
- unm t30834
- unm t30835
- unm_t30834
- unm_t30835
- Type
- protein_coding_gene
- Location
- Chr: 21 Mapping Details/Browsers
- Description
- Predicted to enable actin filament binding activity and microfilament motor activity. Acts upstream of or within several processes, including endocytic recycling; growth involved in heart morphogenesis; and intestinal lipid absorption. Predicted to be located in Schaffer collateral - CA1 synapse. Predicted to be part of myosin complex. Predicted to be active in actin cytoskeleton; cytoplasm; and membrane. Is expressed in several structures, including EVL; gut; periderm; pronephric duct; and sensory system. Used to study microvillus inclusion disease. Human ortholog(s) of this gene implicated in microvillus inclusion disease and progressive familial intrahepatic cholestasis. Orthologous to human MYO5B (myosin VB).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 8 figures from 3 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- cb1077 (11 images)
Wild Type Expression Summary
- All Phenotype Data
- 30 figures from 8 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| sa3127 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa37296 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa43638 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| t30834 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| t30835 | Allele with one point mutation | Unknown | Exon Loss of Exon 10, Frameshift, Premature Stop | ENU | |
| tk34a | unknown | Unknown | Unknown | ENU | |
| tk38 | unknown | Unknown | Unknown | ENU | |
| tt221 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| uq23ks | Allele with one point mutation | Unknown | Frameshift, Premature Stop | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| MO1-myo5b | N/A | (3) |
| MO2-myo5b | N/A | Sonal et al., 2014 |
| MO3-myo5b | N/A | (2) |
| MO4-myo5b | N/A | Gupta et al., 2022 |
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Human Disease
| Disease Ontology Term | Multi-Species Data | OMIM Term | OMIM Phenotype ID |
|---|---|---|---|
| microvillus inclusion disease | Alliance | Diarrhea 2, with microvillus atrophy, with or without cholestasis | 251850 |
| Cholestasis, progressive familial intrahepatic, 10 | 619868 |
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| Human Disease | Fish | Conditions | Citations |
|---|---|---|---|
| microvillus inclusion disease | myo5bt30834/t30834 | standard conditions | Sidhaye et al., 2016 |
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Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Class V myosin, motor domain | Dilute domain | IQ motif, EF-hand binding site | Kinesin motor domain superfamily | Myosin 5b, cargo-binding domain | Myosin head, motor domain-like | Myosin, N-terminal, SH3-like | P-loop containing nucleoside triphosphate hydrolase |
|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:A0A8M9PIE0 | InterPro | 1191 | ||||||||
| UniProtKB:A0A8M3ALG0 | InterPro | 1838 | ||||||||
| UniProtKB:A0A8M9PCR9 | InterPro | 1814 | ||||||||
| UniProtKB:A0A8M9P3E4 | InterPro | 1840 | ||||||||
| UniProtKB:A0A8M9PQJ8 | InterPro | 1840 |
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Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH73-112L6 | ZFIN Curated Data | |
| Contained in | BAC | CH73-203I3 | ZFIN Curated Data | |
| Contained in | BAC | CH211-257I19 | ZFIN Curated Data | |
| Encodes | EST | cb1077 | Thisse et al., 2001 |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001161632 (1) | 5661 nt | ||
| Genomic | GenBank:BX530035 | 179729 nt | ||
| Polypeptide | UniProtKB:A0A8M9P3E4 (1) | 1840 aa |
- Gupta, K., Mukherjee, S., Sen, S., Sonawane, M. (2022) Coordinated activities of Myosin Vb isoforms and mTOR signaling regulate epithelial cell morphology during development. Development (Cambridge, England). 149(6)
- Phatak, M., Kulkarni, S., Miles, L.B., Anjum, N., Dworkin, S., Sonawane, M. (2021) Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish. PLoS Genetics. 17:e1009823
- Oralová, V., Rosa, J.T., Larionova, D., Witten, P.E., Huysseune, A. (2020) Multiple epithelia are required to develop teeth deep inside the pharynx. Proceedings of the National Academy of Sciences of the United States of America. 117(21):11503-11512
- Grassini, D.R., Da Silva, J., Hall, T.E., Baillie, G.J., Simons, C., Parton, R.G., Hogan, B.M., Smith, K.A. (2019) Myosin Vb is required for correct trafficking of N-cadherin and cardiac chamber ballooning. Developmental Dynamics : an official publication of the American Association of Anatomists. 248(4):284-295
- Pinto, C.S., Khandekar, A., Bhavna, R., Kiesel, P., Pigino, G., Sonawane, M. (2019) Microridges are apical epithelial projections formed of F-actin networks that organize the glycan layer. Scientific Reports. 9:12191
- Bayés, À., Collins, M.O., Reig-Viader, R., Gou, G., Goulding, D., Izquierdo, A., Choudhary, J.S., Emes, R.D., Grant, S.G. (2017) Evolution of complexity in the zebrafish synapse proteome. Nature communications. 8:14613
- Sidhaye, J., Pinto, C.S., Dharap, S., Jacob, T., Bhargava, S., Sonawane, M. (2016) The zebrafish goosepimples/myosin Vb mutant exhibits cellular attributes of human microvillus inclusion disease. Mechanisms of Development. 142:62-74
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Alvers, A.L., Ryan, S., Scherz, P.J., Huisken, J., and Bagnat, M. (2014) Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling. Development (Cambridge, England). 141(5):1110-1119
- Sonal, ., Sidhaye, J., Phatak, M., Banerjee, S., Mulay, A., Deshpande, O., Bhide, S., Jacob, T., Gehring, I., Nuesslein-Volhard, C., Sonawane, M. (2014) Myosin Vb Mediated Plasma Membrane Homeostasis Regulates Peridermal Cell Size and Maintains Tissue Homeostasis in the Zebrafish Epidermis. PLoS Genetics. 10:e1004614
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