Gene
lars1b
- ID
- ZDB-GENE-030114-7
- Name
- leucyl-tRNA synthetase 1b
- Symbol
- lars1b Nomenclature History
- Previous Names
-
- lars
- larsb
- cb667 (1)
- wu:fb36b12
- Type
- protein_coding_gene
- Location
- Chr: 21 Mapping Details/Browsers
- Description
- Predicted to enable leucine-tRNA ligase activity. Acts upstream of or within liver development; regulation of autophagy; and swimming behavior. Is expressed in several structures, including alar plate midbrain region; brain; digestive system; mesoderm; and musculature system. Used to study infantile liver failure syndrome 1. Human ortholog(s) of this gene implicated in infantile liver failure syndrome 1. Orthologous to human LARS1 (leucyl-tRNA synthetase 1).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 8 figures from 3 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- cb667 (30 images)
Wild Type Expression Summary
- All Phenotype Data
- 12 figures from 3 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| cq68 | Allele with one delins | Unknown | Frameshift, Premature Stop | CRISPR | |
| oi3 | Allele with one deletion | Exon 2 | Unknown | CRISPR | |
| oi12 | Allele with one MNV | Unknown | Unknown | CRISPR | |
| sa15597 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa39348 | Allele with one point mutation | Unknown | Splice Site | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-lars1b | Wang et al., 2018 | |
| CRISPR2-lars1b | Inoue et al., 2021 | |
| CRISPR3-lars1b | Inoue et al., 2024 |
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Human Disease
| Disease Ontology Term | Multi-Species Data | OMIM Term | OMIM Phenotype ID |
|---|---|---|---|
| infantile liver failure syndrome 1 | Alliance | ?Infantile liver failure syndrome 1 | 615438 |
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| Human Disease | Fish | Conditions | Citations |
|---|---|---|---|
| infantile liver failure syndrome 1 | lars1boi12/oi12 (AB) | standard conditions | Inoue et al., 2024 |
| infantile liver failure syndrome 1 | lars1bcq68/cq68 | standard conditions | Wang et al., 2018 |
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Domain, Family, and Site Summary
| Type | InterPro ID | Name |
|---|---|---|
| Conserved_site | IPR001412 | Aminoacyl-tRNA synthetase, class I, conserved site |
| Domain | IPR002300 | Aminoacyl-tRNA synthetase, class Ia |
| Domain | IPR013155 | Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding |
| Domain | IPR054509 | Leucine--tRNA ligase, ubiquitin-like domain |
| Domain | IPR055416 | Leucine--tRNA ligase, RagD-binding domain |
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Domain Details Per Protein
| Protein | Additional Resources | Length | Aminoacyl-tRNA synthetase, class Ia | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding | Aminoacyl-tRNA synthetase, class I, conserved site | Leucine--tRNA ligase, RagD-binding domain | Leucine--tRNA ligase, ubiquitin-like domain | Leucyl-tRNA synthetase, class Ia, archaeal/eukaryotic cytosolic | Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding | Rossmann-like alpha/beta/alpha sandwich fold | Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain |
|---|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:A0A8N7T837 | InterPro | 1176 |
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| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
lars1b-201
(1)
|
Ensembl | 5,362 nt | ||
| mRNA |
larsb-202
(1)
|
Ensembl | 2,973 nt |
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Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | DKEY-83D6 | ZFIN Curated Data | |
| Encodes | EST | cb667 | Thisse et al., 2001 | |
| Encodes | EST | fb36b12 |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:XM_693187 (1) | 4354 nt | ||
| Genomic | GenBank:CU302322 (1) | 218024 nt | ||
| Polypeptide | UniProtKB:A0A8N7T837 (1) | 1176 aa |
- Inoue, M., Sebastian, W.A., Sonoda, S., Miyahara, H., Shimizu, N., Shiraishi, H., Maeda, M., Yanagi, K., Kaname, T., Hanada, R., Hanada, T., Ihara, K. (2024) Biallelic variants in LARS1 induce steatosis in developing zebrafish liver via enhanced autophagy. Orphanet journal of rare diseases. 19:219219
- Inoue, M., Miyahara, H., Shiraishi, H., Shimizu, N., Tsumori, M., Kiyota, K., Maeda, M., Umeda, R., Ishitani, T., Hanada, R., Ihara, K., Hanada, T. (2021) Leucyl-tRNA synthetase deficiency systemically induces excessive autophagy in zebrafish. Scientific Reports. 11:8392
- Lin, S.J., Vona, B., Barbalho, P.G., Kaiyrzhanov, R., Maroofian, R., Petree, C., Severino, M., Stanley, V., Varshney, P., Bahena, P., Alzahrani, F., Alhashem, A., Pagnamenta, A.T., Aubertin, G., Estrada-Veras, J.I., Hernández, H.A.D., Mazaheri, N., Oza, A., Thies, J., Renaud, D.L., Dugad, S., McEvoy, J., Sultan, T., Pais, L.S., Tabarki, B., Villalobos-Ramirez, D., Rad, A., Genomics England Research Consortium, Galehdari, H., Ashrafzadeh, F., Sahebzamani, A., Saeidi, K., Torti, E., Elloumi, H.Z., Mora, S., Palculict, T.B., Yang, H., Wren, J.D., Ben Fowler, ., Joshi, M., Behra, M., Burgess, S.M., Nath, S.K., Hanna, M.G., Kenna, M., Merritt, J.L., Houlden, H., Karimiani, E.G., Zaki, M.S., Haaf, T., Alkuraya, F.S., Gleeson, J.G., Varshney, G.K. (2021) Biallelic variants in KARS1 are associated with neurodevelopmental disorders and hearing loss recapitulated by the knockout zebrafish. Genetics in medicine : official journal of the American College of Medical Genetics. 23(10):1933-1943
- Maugars, G., Nourizadeh-Lillabadi, R., Weltzien, F.A. (2020) New Insights Into the Evolutionary History of Melatonin Receptors in Vertebrates, With Particular Focus on Teleosts. Frontiers in endocrinology. 11:538196
- Wang, Z., Song, J., Luo, L., Ma, J. (2018) Loss of Leucyl-tRNA synthetase b leads to ILFS1-like symptoms in zebrafish. Biochemical and Biophysical Research Communications. 505(2):378-384
- Bayés, À., Collins, M.O., Reig-Viader, R., Gou, G., Goulding, D., Izquierdo, A., Choudhary, J.S., Emes, R.D., Grant, S.G. (2017) Evolution of complexity in the zebrafish synapse proteome. Nature communications. 8:14613
- Waldron, A.L., Cahan, S.H., Franklyn, C.S., Ebert, A.M. (2017) A single Danio rerio hars gene encodes both cytoplasmic and mitochondrial histidyl-tRNA synthetases. PLoS One. 12:e0185317
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Recher, G., Jouralet, J., Brombin, A., Heuzé, A., Mugniery, E., Hermel, J.M., Desnoulez, S., Savy, T., Herbomel, P., Bourrat, F., Peyriéras, N., Jamen, F., and Joly, J.S. (2013) Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis. Development (Cambridge, England). 140(24):4860-9
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