Gene
robo4
- ID
- ZDB-GENE-020809-1
- Name
- roundabout, axon guidance receptor, homolog 4 (Drosophila)
- Symbol
- robo4 Nomenclature History
- Previous Names
-
- sc:d0823
- wu:fa16f09
- Type
- protein_coding_gene
- Location
- Chr: 10 Mapping Details/Browsers
- Description
- Predicted to enable cell-cell adhesion mediator activity. Acts upstream of or within several processes, including plasma membrane bounded cell projection assembly; positive regulation of Rac protein signal transduction; and positive regulation of cell-matrix adhesion. Located in plasma membrane. Is expressed in several structures, including cardiovascular system; central nervous system; neural tube; optic cup; and vestibuloauditory system. Human ortholog(s) of this gene implicated in aortic valve disease 3. Orthologous to human ROBO4 (roundabout guidance receptor 4).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 23 figures from 13 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- cssl:d823 (24 images)
Wild Type Expression Summary
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| jh176 | Allele with one deletion | Unknown | Unknown | CRISPR | |
| sa6175 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa8702 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa21760 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa24878 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa38805 | Allele with one point mutation | Unknown | Premature Stop | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-robo4 | Gould et al., 2019 | |
| MO1-robo4 | N/A | (3) |
| MO2-robo4 | N/A | (2) |
| MO3-robo4 | N/A | Fish et al., 2011 |
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Human Disease
| Disease Ontology Term | Multi-Species Data | OMIM Term | OMIM Phenotype ID |
|---|---|---|---|
| aortic valve disease 3 | Alliance | Aortic valve disease 3 | 618496 |
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Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Fibronectin type III | Fibronectin type III superfamily | Immunoglobulin domain subtype | Immunoglobulin I-set | Immunoglobulin-like domain | Immunoglobulin-like domain superfamily | Immunoglobulin-like fold | Immunoglobulin subtype 2 |
|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:A4JYG5 | InterPro | 591 | ||||||||
| UniProtKB:A0A8M2BK13 | InterPro | 1093 | ||||||||
| UniProtKB:F1RE66 | InterPro | 1134 |
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Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-164N21 | ZFIN Curated Data | |
| Contained in | BAC | DKEY-30J19 | ZFIN Curated Data | |
| Contains | SNP | rs3728312 | ZFIN Curated Data | |
| Contains | SNP | rs3728313 | ZFIN Curated Data | |
| Encodes | EST | fa16f09 | ZFIN Curated Data | |
| Encodes | cDNA | cssl:d823 | Bushell et al., 2007 |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:XM_684163 (1) | 5203 nt | ||
| Genomic | GenBank:AL845320 (1) | 183417 nt | ||
| Polypeptide | UniProtKB:F1RE66 (1) | 1134 aa |
- Ezhkova, D., Schwarzer, S., Spieß, S., Geffarth, M., Machate, A., Zöller, D., Stucke, J., Alexopoulou, D., Lesche, M., Dahl, A., Hans, S. (2023) Transcriptome analysis reveals an Atoh1b-dependent gene set downstream of Dlx3b/4b during early inner ear development in zebrafish. Biology Open. 12(6):
- Lazzaroni, F., Meessen, J.M.T.A., Sun, Y., Lanfranconi, S., Scola, E., D'Alessandris, Q.G., Tassi, L., Carriero, M.R., Castori, M., Marino, S., Blanda, A., Nicolis, E.B., Novelli, D., Calabrese, R., Agnelli, N.M., Bottazzi, B., Leone, R., Mazzola, S., Besana, S., Catozzi, C., Nezi, L., Lampugnani, M.G., Malinverno, M., Grdseloff, N., Rödel, C.J., Rezai Jahromi, B., Bolli, N., Passamonti, F., Magnusson, P.U., Abdelilah-Seyfried, S., Dejana, E., Latini, R. (2023) Circulating biomarkers in familial cerebral cavernous malformation. EBioMedicine. 99:104914104914
- Zhou, L., Zhang, W., Xiang, Y., Qian, Z., Zhou, J., Ni, L., Feng, Y., Gao, B. (2023) The apatinib and pemetrexed combination has antitumor and antiangiogenic effects against NSCLC. Open life sciences. 18:2022053320220533
- Martin, A., Babbitt, A., Pickens, A.G., Pickett, B.E., Hill, J.T., Suli, A. (2022) Single-Cell RNA Sequencing Characterizes the Molecular Heterogeneity of the Larval Zebrafish Optic Tectum. Frontiers in molecular neuroscience. 15:818007
- Chen, K., Fan, Y., Gu, J., Han, Z., Zeng, H., Mao, C., Wang, C. (2020) In vivo Screening of Natural Products Against Angiogenesis and Mechanisms of Anti-Angiogenic Activity of Deoxysappanone B 7,4'-Dimethyl Ether. Drug design, development and therapy. 14:3069-3078
- Gould, R.A., Aziz, H., Woods, C.E., Seman-Senderos, M.A., Sparks, E., Preuss, C., Wünnemann, F., Bedja, D., Moats, C.R., McClymont, S.A., Rose, R., Sobreira, N., Ling, H., MacCarrick, G., Kumar, A.A., Luyckx, I., Cannaerts, E., Verstraeten, A., Björk, H.M., Lehsau, A.C., Jaskula-Ranga, V., Lauridsen, H., Shah, A.A., Bennett, C.L., Ellinor, P.T., Lin, H., Isselbacher, E.M., Lino Cardenas, C.L., Butcher, J.T., Hughes, G.C., Lindsay, M.E., Baylor-Hopkins Center for Mendelian Genomics, MIBAVA Leducq Consortium, Mertens, L., Franco-Cereceda, A., Verhagen, J.M.A., Wessels, M., Mohamed, S.A., Eriksson, P., Mital, S., Van Laer, L., Loeys, B.L., Andelfinger, G., McCallion, A.S., Dietz, H.C. (2019) ROBO4 variants predispose individuals to bicuspid aortic valve and thoracic aortic aneurysm. Nature Genetics. 51:42-50
- Lu, T., Zhang, T., Wang, C., Yang, N., Pan, Y.H., Dang, S., Zhang, W. (2019) Adamts18 deficiency in zebrafish embryo causes defective trunk angiogenesis and caudal vein plexus formation. Biochemical and Biophysical Research Communications. 521(4):907-913
- Chen, K., Wang, C., Fan, Y., Gu, J., Han, Z., Wang, Y., Gao, L., Zeng, H. (2018) Identification of mundoserone by zebrafish in vivo screening as a natural product with anti-angiogenic activity. Experimental and Therapeutic Medicine. 16:4562-4568
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Takeuchi, M., Yamaguchi, S., Yonemura, S., Kakiguchi, K., Sato, Y., Higashiyama, T., Shimizu, T., Hibi, M. (2015) Type IV Collagen Controls the Axogenesis of Cerebellar Granule Cells by Regulating Basement Membrane Integrity in Zebrafish. PLoS Genetics. 11:e1005587
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