Gene
gbx2
- ID
- ZDB-GENE-020509-2
- Name
- gastrulation brain homeobox 2
- Symbol
- gbx2 Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 6 Mapping Details/Browsers
- Description
- Enables DNA-binding transcription factor activity, RNA polymerase II-specific. Acts upstream of or within cerebellum development; iridophore differentiation; and telencephalon regionalization. Predicted to be active in nucleus. Is expressed in several structures, including midbrain hindbrain boundary neural keel; midbrain hindbrain boundary neural rod; midbrain neural rod; nervous system; and presumptive rhombomere 1. Orthologous to human GBX2 (gastrulation brain homeobox 2).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 40 figures from 23 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| e30 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| fh248 | Allele with one point mutation | Unknown | Unknown | ENU | |
| fh253 | Allele with one point mutation | Unknown | Premature Stop | not specified | |
| gbx2_unrecovered | Allele with one point mutation | Unknown | Unknown | ENU | |
| mpn433Tg | Transgenic insertion | Unknown | Unknown | DNA and CRISPR | |
| sa40660 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| tud51Tg | Transgenic insertion | Unknown | Unknown | DNA and CRISPR |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-gbx2 | Kesavan et al., 2020 | |
| CRISPR2-gbx2 | Zhao et al., 2024 | |
| MO1-gbx2 | N/A | (4) |
| MO2-gbx2 | N/A | Seifinejad et al., 2019 |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Homedomain-like superfamily | Homeobox, conserved site | Homeobox protein GBX-1/2 | Homeodomain | Homeodomain, metazoa |
|---|---|---|---|---|---|---|---|
| UniProtKB:Q8QGU1 | InterPro | 342 |
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Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg1(gbx2-hsp70l:EGFP) |
|
| 1 | (5) | |
| Tg2(gbx2-hsp70l:EGFP) |
|
| 1 | (3) | |
| Tg3(gbx2-hsp70l:EGFP) |
|
| 1 | (2) | |
| Tg4(gbx2-hsp70l:EGFP) |
|
| 1 | (3) | |
| Tg(gbx2:Venus-NLS) |
|
| 1 | Kesavan et al., 2020 | |
| Tg(hsp70l:gbx2) |
|
| 1 | (4) | |
| Tg(mitfa:gbx2,EGFP) |
|
| 1 | Jang et al., 2021 |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | DKEY-30F11 | ZFIN Curated Data | |
| Encodes | EST | fd07f12 | ZFIN Curated Data | |
| Encodes | EST | mb2 | Rhinn et al., 2003 | |
| Encodes | cDNA | MGC:73023 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_152964 (1) | 2113 nt | ||
| Genomic | GenBank:CR847983 (2) | 199111 nt | ||
| Polypeptide | UniProtKB:Q8QGU1 (1) | 342 aa |
- Zebrafish Nomenclature Committee (2025) Nomenclature Data Curation (2025). Nomenclature Committee Submission.
- Maekawa, M., Saito, S., Isobe, D., Takemoto, K., Miura, Y., Dobashi, Y., Yamasu, K. (2024) The Oct4-related PouV gene, pou5f3, mediates isthmus development in zebrafish by directly and dynamically regulating pax2a. Cells & development. 179:203933
- Zebrafish Nomenclature Committee (2024) Nomenclature Data Curation (2024). Nomenclature Committee Submission.
- Zhao, S., Wang, C., Luo, H., Li, F., Wang, Q., Xu, J., Huang, Z., Liu, W., Zhang, W. (2024) A role for Retinoblastoma 1 in hindbrain morphogenesis by regulating GBX family. Journal of genetics and genomics = Yi chuan xue bao. 51(9):900-910
- Shainer, I., Kuehn, E., Laurell, E., Al Kassar, M., Mokayes, N., Sherman, S., Larsch, J., Kunst, M., Baier, H. (2023) A single-cell resolution gene expression atlas of the larval zebrafish brain. Science advances. 9:eade9909
- Brożko, N., Baggio, S., Lipiec, M.A., Jankowska, M., Szewczyk, Ł.M., Gabriel, M.O., Chakraborty, C., Ferran, J.L., Wiśniewska, M.B. (2022) Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish. Frontiers in Neuroanatomy. 16:838567
- Zhou, J., Yang, Y.J., Gan, R.H., Wang, Y., Li, Z., Zhang, X.J., Gui, J.F., Zhou, L. (2022) Foxl2a and Foxl2b are involved in midbrain-hindbrain boundary development in zebrafish. Gene expression patterns : GEP. 46:119286
- Jang, H.S., Chen, Y., Ge, J., Wilkening, A.N., Hou, Y., Lee, H.J., Choi, Y.R., Lowdon, R.F., Xing, X., Li, D., Kaufman, C.K., Johnson, S.L., Wang, T. (2021) Epigenetic dynamics shaping melanophore and iridophore cell fate in zebrafish. Genome biology. 22:282
- Zimmer, T.S., Korotkov, A., Zwakenberg, S., Jansen, F.E., Zwartkruis, F.J.T., Rensing, N.R., Wong, M., Mühlebner, A., van Vliet, E.A., Aronica, E., Mills, J.D. (2021) Upregulation of the pathogenic transcription factor SPI1/PU.1 in tuberous sclerosis complex and focal cortical dysplasia by oxidative stress. Brain pathology (Zurich, Switzerland). 31(5):e12949
- Kesavan, G., Machate, A., Hans, S., Brand, M. (2020) Cell-fate plasticity, adhesion and cell sorting complementarily establish a sharp midbrain-hindbrain boundary. Development (Cambridge, England). 147(11):
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