Gene
nkx2.4b
- ID
- ZDB-GENE-000830-1
- Name
- NK2 homeobox 4b
- Symbol
- nkx2.4b Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 20 Mapping Details/Browsers
- Description
- Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within hypothalamus development and thyroid gland development. Predicted to be active in nucleus. Is expressed in several structures, including endocrine system; forebrain; neural keel; neural plate; and swim bladder. Orthologous to human NKX2-4 (NK2 homeobox 4).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 81 figures from 52 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
Phenotype Summary
Mutations
| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-nkx2.4b | Trubiroha et al., 2018 | |
| CRISPR2-nkx2.4b | Trubiroha et al., 2018 | |
| MO1-nkx2.4b | N/A | (3) |
| MO2-nkx2.4b | N/A | Elsalini et al., 2003 |
| TALEN1-nkx2.4b | Schredelseker et al., 2020 | |
| TALEN2-nkx2.4b | Hutcheson et al., 2020 |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Homedomain-like superfamily | Homeobox, conserved site | Homeobox NK-like transcription regulators | Homeodomain | Homeodomain, metazoa |
|---|---|---|---|---|---|---|---|
| UniProtKB:Q9I8L7 | InterPro | 307 |
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| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
nkx2.4b-201
(1)
|
Ensembl | 1,151 nt |
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Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg(nkx2.4b:GFP,myl7:EGFP) |
|
| 1 | Hutcheson et al., 2020 |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | DKEY-165F9 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:193896 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:193913 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_131589 (1) | 1175 nt | ||
| Genomic | GenBank:CT573061 (2) | 79800 nt | ||
| Polypeptide | UniProtKB:Q9I8L7 (1) | 307 aa |
- Fang, Y., Wan, J.P., Wang, Z., Song, S.Y., Zhang, C.X., Yang, L., Zhang, Q.Y., Yan, C.Y., Wu, F.Y., Lu, S.Y., Sun, F., Han, B., Zhao, S.X., Dong, M., Song, H.D. (2024) Deficiency of the HGF/Met pathway leads to thyroid dysgenesis by impeding late thyroid expansion. Nature communications. 15:31653165
- Umeda, K., Tanaka, K., Chowdhury, G., Nasu, K., Kuroyanagi, Y., Yamasu, K. (2024) Evolutionarily conserved roles of foxg1a in the developing subpallium of zebrafish embryos. Development, growth & differentiation. 66(3):219-234
- Liu, J., Yang, L., Lu, Z., Wang, Q. (2023) Tmem88 plays an essential role in pharyngeal pouch progenitor specification by inhibiting Wnt/β-catenin signaling. Life medicine. 2:lnad044lnad044
- Chu, S., Kwon, B.R., Lee, Y.M., Zoh, K.D., Choi, K. (2021) Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio). Chemosphere. 263:128176
- Kim, J., Lee, G., Lee, Y.M., Zoh, K.D., Choi, K. (2021) Thyroid disrupting effects of perfluoroundecanoic acid and perfluorotridecanoic acid in zebrafish (Danio rerio) and rat pituitary (GH3) cell line. Chemosphere. 262:128012
- Yan, Y.L., Titus, T., Desvignes, T., BreMiller, R., Batzel, P., Sydes, J., Farnsworth, D., Dillon, D., Wegner, J., Phillips, J.B., Peirce, J., Dowd, J., Undiagnosed Diseases Network, Buck, C.L., Miller, A., Westerfield, M., Postlethwait, J.H. (2021) A fish with no sex: gonadal and adrenal functions partition between zebrafish NR5A1 co-orthologs. Genetics. 217(2)
- Bando, H., Gergics, P., Bohnsack, B.L., Toolan, K.P., Richter, C.E., Shavit, J.A., Camper, S.A. (2020) otx2b mutant zebrafish have pituitary, eye and mandible defects that model mammalian disease. Human molecular genetics. 29(10):1648-1657
- Chen, X., Teng, M., Zhang, J., Qian, L., Duan, M., Cheng, Y., Zhao, F., Zheng, J., Wang, C. (2020) Tralopyril induces developmental toxicity in zebrafish embryo (Danio rerio) by disrupting the thyroid system and metabolism. The Science of the total environment. 746:141860
- Hutcheson, D.A., Xie, Y., Figueroa, P., Dorsky, R.I. (2020) A transgene targeted to the zebrafish nkx2.4b locus drives specific GFP expression and disrupts thyroid development. Developmental Dynamics : an official publication of the American Association of Anatomists. 249(11):1387-1393
- Kim, S., Stroski, K.M., Killeen, G., Smitherman, C., Simcik, M.F., Brooks, B.W. (2020) 8:8 Perfluoroalkyl phosphinic acid affects neurobehavioral development, thyroid disruption, and DNA methylation in developing zebrafish. The Science of the total environment. 736:139600
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