FIGURE

Fig. 3

ID

ZDB-FIG-250114-17

Publication

Horn et al., 2025 - Isotonic medium treatment limits burn wound microbial colonisation and improves tissue repair

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Fig. 3

Isotonic medium treatment reduces microbial colonisation of burned tissue. (A) Unwounded or burn wounded Tg(Krt4-UtrCH-GFP) larval zebrafish, labelling epithelial keratinocytes, after 1-h exposure to RFP-tagged C. albicans. Dashed box shows area of inset to the right. Scale bar = 500 μm (whole larvae) and 50 μm (inset). (B) C. albicans colonisation of control and isotonic medium treated tissue 1 hpb. (C) Quantification of C. albicans colonisation 1 hpb. N = 21 control and 18 isotonic treated larvae per condition. (D) mNeon-tagged C. albicans colonisation of Tg(LyzC-BFPxMpeg1.1-mCherry) larvae 24 hpb. Dashed line indicates tailfin boundary. (E–G) Quantification of larvae burned as in D showing C. albicans colonisation (E, N ≥ 26 control and 27 isotonic treated larvae), neutrophil recruitment (F, N ≥ 20 control and 22 isotonic treated larvae) and macrophage recruitment (G, N ≥ 14 control and 19 isotonic treated larvae) over time. Scale bar = 50 μm in B, D. Asterisk indicates p < 0.05 by two-tailed Mann–Whitney test (C) and two-way ANOVA (E–G).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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