Enhancer Trap Construct

Et(GAL4-VP16,myl7:GFP)

ID
ZDB-ETCONSTRCT-120604-1
Name
Et(GAL4-VP16,myl7:GFP)
Previous Names
  • Et(Gal4-VP16)
  • Et(bp:Gal4VP16) (1)
  • Et(Gal4-VP16,myl7:GFP)
Type
engineered_plasmid
Regulatory Regions
Coding Sequences
Contains Sequences
None
Note
This enhancer trapping construct is based on pDestTol2CG3, a multisite-Gateway-based plasmid. It contains a minimal adenovirus E1b promoter driving Gal4VP16 (Gal4 DNA-binding domain fused to the VP16 transcriptional activation domain) followed by the late SV40 polyA signal sequence. Following (3’ to) the enhancer trapping construct is a transgene marker: the cmlc2 promoter driving EGFP followed by the SV40 late polyA signal sequence; this marks the heart with green fluorescence. The enhancer trapping construct and the transgene marker are in a tail-to-tail orientation such that they drive transcription in opposite directions. The entire cassette (enhancer trapping construct + transgene marker) is flanked by miniTol2 transposon ends (Balciunas et al., 2006).
Plasmid Map
Genomic Features
That Utilize Et(GAL4-VP16,myl7:GFP) Construct
Genomic Feature Affected Genomic Regions
zc1000aEt
zc1002aEt
zc1003aEt
zc1004aEt
zc1006aEt
1 - 5 of 92 Show all
Transgenics
That Utilize Et(GAL4-VP16,myl7:GFP) Construct
Sequence Information
Other Construct Pages
None
Citations