Enhancer Trap Construct
Et(GAL4-VP16,myl7:GFP)
- ID
- ZDB-ETCONSTRCT-120604-1
- Name
- Et(GAL4-VP16,myl7:GFP)
- Previous Names
-
- Et(Gal4-VP16)
- Et(bp:Gal4VP16) (1)
- Et(Gal4-VP16,myl7:GFP)
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- Contains Sequences
- None
- Note
-
This enhancer trapping construct is based on pDestTol2CG3, a multisite-Gateway-based plasmid. It contains a minimal adenovirus E1b promoter driving Gal4VP16 (Gal4 DNA-binding domain fused to the VP16 transcriptional activation domain) followed by the late SV40 polyA signal sequence. Following (3’ to) the enhancer trapping construct is a transgene marker: the cmlc2 promoter driving EGFP followed by the SV40 late polyA signal sequence; this marks the heart with green fluorescence. The enhancer trapping construct and the transgene marker are in a tail-to-tail orientation such that they drive transcription in opposite directions. The entire cassette (enhancer trapping construct + transgene marker) is flanked by miniTol2 transposon ends (Balciunas et al., 2006).
Plasmid Map
Genomic Features
That Utilize Et(GAL4-VP16,myl7:GFP) Construct
Transgenics
That Utilize Et(GAL4-VP16,myl7:GFP) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| c264Tg/+; zc1000aEt/+ (AB) |
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| c264Tg/+; zc1004aEt/+ (AB) |
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| c264Tg/+; zc1008aEt/+ (AB) |
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| c264Tg/+; zc1009aEt/+ (AB) |
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| c264Tg/+; zc1012aEt/+ (AB) |
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Sequence Information
Other Construct Pages
None
Citations