CRISPR

CRISPR5-ptk7a

ID

ZDB-CRISPR-250402-2

Name

CRISPR5-ptk7a

Previous Names

None

Target

ptk7a (1)

Sequence

5' - TTTATGGACGAGAAGACG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
udm400	ptk7a

Expression

Gene expression in Wild Types + CRISPR5-ptk7a

No data available

Phenotype

Phenotype resulting from CRISPR5-ptk7a

No data available

Phenotype of all Fish created by or utilizing CRISPR5-ptk7a

Phenotype	Fish	Conditions	Figures
vertebra fused with vertebra, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 4 from Wang et al., 2024
notochord inner cell caveola spherical, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 3 from Wang et al., 2024
notochord inner cell has fewer parts of type notochord inner cell caveola, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 3 from Wang et al., 2024
notochord inner cell cell-cell junction loose, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 3 from Wang et al., 2024
notochord inner cell increased volume, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 2 from Wang et al., 2024
notochord has fewer parts of type notochord inner cell, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 2 from Wang et al., 2024
notochord apoptotic process increased process quality, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 2 from Wang et al., 2024
vertebra bone mineralization decreased process quality, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 4 from Wang et al., 2024
notochord anterior region undulate, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 4 from Wang et al., 2024
notochord increased width, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 1 from Wang et al., 2024
notochord inner cell desmosome malformed, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 3 from Wang et al., 2024
convergent extension involved in notochord morphogenesis decreased process quality, abnormal	ptk7a^{udm400/udm400}; udm401Tg	standard conditions	Fig. 1 from Wang et al., 2024

Citations