CRISPR

CRISPR1-klc4

ID

ZDB-CRISPR-231212-3

Name

CRISPR1-klc4

Previous Names

None

Target

klc4 (1)

Sequence

5' - GAGCGTGGCACAGCTGGAGGAGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
uw314	klc4

Expression

Gene expression in Wild Types + CRISPR1-klc4

No data available

Phenotype

Phenotype resulting from CRISPR1-klc4

No data available

Phenotype of all Fish created by or utilizing CRISPR1-klc4

Phenotype	Fish	Conditions	Figures
Rohon-Beard neuron regulation of microtubule depolymerization decreased process quality, abnormal	klc4^uw314/uw314 (AB)	standard conditions	Figure 10 from Haynes et al., 2022
exploration behavior decreased process quality, abnormal	klc4^uw314/uw314 (AB)	standard conditions	Figure 11 , Figure 12 from Haynes et al., 2022
brain klc4 expression absent, abnormal	klc4^uw314/uw314 (AB)	standard conditions	Figure 1 from Haynes et al., 2022
spinal cord klc4 expression absent, abnormal	klc4^uw314/uw314 (AB)	standard conditions	Figure 1 from Haynes et al., 2022
Rohon-Beard neuron vesicle-mediated transport between endosomal compartments decreased process quality, abnormal	klc4^uw314/uw314 (AB)	standard conditions	Figure 8 from Haynes et al., 2022
thigmotaxis increased process quality, abnormal	klc4^uw314/uw314 (AB)	standard conditions	Figure 11 from Haynes et al., 2022
Rohon-Beard neuron collateral sprouting decreased occurrence, abnormal	klc4^uw314/uw314 (AB)	standard conditions	Figure 2 from Haynes et al., 2022
Rohon-Beard neuron tubulin N-acetyltransferase activity decreased occurrence, abnormal	klc4^uw314/uw314 (AB)	standard conditions	Figure 10 from Haynes et al., 2022
Rohon-Beard neuron microtubule polymerization increased process quality, abnormal	klc4^uw314/uw314 (AB)	standard conditions	Figure 9 from Haynes et al., 2022
Rohon-Beard neuron axonal growth cone posterior orientation, abnormal	klc4^uw314/uw314; uw4Tg (AB)	standard conditions	Figure 4 , Figure 5 from Haynes et al., 2022
Rohon-Beard neuron axon lateral to Rohon-Beard neuron cell body, abnormal	klc4^uw314/uw314; uw4Tg (AB)	standard conditions	Figure 7 from Haynes et al., 2022
Rohon-Beard neuron dendrite self-avoidance decreased occurrence, abnormal	klc4^uw314/uw314; uw4Tg (AB)	standard conditions	Figure 5 from Haynes et al., 2022
Rohon-Beard neuron fasciculation of sensory neuron axon increased occurrence, abnormal	klc4^uw314/uw314; uw4Tg (AB)	standard conditions	Figure 6 from Haynes et al., 2022
Rohon-Beard neuron collateral sprouting decreased occurrence, abnormal	klc4^uw314/uw314; uw4Tg (AB)	standard conditions	Figure 2 from Haynes et al., 2022
Rohon-Beard neuron axon extension involved in axon guidance process quality, abnormal	klc4^uw314/uw314; uw4Tg (AB)	standard conditions	Figure 7 from Haynes et al., 2022

Citations